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| Product title : | Determination of bovine rotavirus G genotypes in Kashmir, India |
| Author(s) : | S.A. Wani, M.A. Bhat, S.M. Ishaq & M.A. Ashrafi |
Summary :Rotavirus ribonucleic acid (RNA) was extracted from ten faecal samples of diarrhoeic calves positive for group A rotavirus by enzyme-linked immunosorbent assay (ELISA). A portion of the extracted RNA was run in polyacrylamide gel to determine the presence of rotaviral RNA and the rest subjected to reverse transcriptase-polymerase chain reaction (RT-PCR) to generate the near full length VP7 gene. Only six samples yielded the desired product. The amplified products were subjected to G-typing by PCR using a cocktail of G6, G8 and G10 typing primers. All of the six samples were characterised as G10 and none of the samples revealed mixed infection by twin G types. Four samples, despite possessing sufficient rotavirus particles as revealed by ELISA and polyacrylamide gel electrophoresis, did not yield any amplified product on RT-PCR. This could be due to non-specific inhibitors of the PCR reaction, present in the faecal samples, being carried through the extraction procedures. | |