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|Author(s) :||K.M. Osman, M.I. El-Enbaawy, N.A. Ezzeldeen & H.M.G. Hussein|
Although Clostridium perfringens is recognised as an important cause ofclostridial enteric diseases, there is only limited knowledge about the association of particular C. perfringens toxinotypes (types A to E) with mastitis in domestic animals. In this study, mastitis was detected in 213/623 (34.12%) and 8/83 (9.64%) of the quarter milk samples collected from cases of clinical mastitis in cows and buffalo, respectively. The micro-organism was isolated in an incidence of 16/357 (4.48%) of milk samples from cows and 1/25 (4.0%) of samples from buffalo. Infection in one quarter was the most typical situation found (83%in cows and 87% in buffalo). Clostridium perfringens infection was also correlated to the season, with the highest proportion of isolates being found during spring (10.71%) and winter (7.07%). Using the classical toxin neutralisation typing method, 17 strains, isolated from cow and buffalo milk, were identified as C. perfringens type A, and selected for molecular analysis.
α/cpa gene – Alpha-toxin – Bovines – Buffalo – Clostridium perfringens – Dairy cattle– Egypt – Mastitis.